Coding

Part:BBa_K4192020:Design

Designed by: Muhua Liu   Group: iGEM22_CAU_China   (2022-09-29)


obcA, one of the genes required for oxalic acid synthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1328
    Illegal XhoI site found at 91
    Illegal XhoI site found at 1215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 184
    Illegal NgoMIV site found at 176
    Illegal NgoMIV site found at 507
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1578
    Illegal AgeI site found at 113
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When aimed at producing oxalic acid, you may want it work together with obcB to increase the concentration


Source

It is origin from the genomic sequence of Burkholderia glumae

References